Evaluación Mutagénica de las tabletas orales de PV-2 con ensayos in vivo
nº37 [Diciembre 11 - Febrero 12] Retel04/03/2012
Yesenia Rivero Salgado, Ailemys Curbelo Valiente, Gladys Pérez Arnáez, Nidia Fernández Esperón, Isidoro Scull Campos, Antonia C. Remigio Montero, Axel Mancebo Rodríguez. Asniel F. García Díaz,
Centro Nacional para la Producción de Animales de Laboratorio, CENPALAB. Finca Tirabeque, Carretera Cacahual Km 21/2, Bejucal, La Habana Cuba. Correo yesenia@cenpalab.inf.cu
Resumen
Las tabletas orales PV-2, están realizadas con tintura de la planta Morinda royoc L., nombrada comúnmente en nuestro país como “Garañon”, lo que confiere efectos estimulantes, vigorizaste y estimulador de la libido. El desarrollo de estos productos tiene necesariamente que llevar en paralelo un sistema evaluativo que permita conocer con certeza el impacto que sobre la salud humana, animal y el medio ambiente puedan ocasionar tales productos naturales. Se realizó el estudio del efecto mutagénico en ratones y ratas mediante los Ensayos de Aberración Cromosómica, Micronúcleos y Espermatozoides administrados por vía oral, empleando como controles positivo la acrilamida (20 mg/kg p.c) y la ciclofosfamida (40 mg/kg p.c), y como control negativo el agua estéril. Al final del tratamiento los animales fueron sacrificados y procesado para obtener las preparaciones según el procedimiento. Los resultados demuestran la no evidencia de efectos clastogénicos en la médula ósea de ratón, ni efecto mutagénico sobre los cromosomas de la médula ósea de las ratas. No mostró tampoco efectos genotóxicos ni citotóxicos sobre las células espermáticas del ratón. Estos resultados amplían los conocimientos sobre la inocuidad de este producto en su relación con el hombre.
Palabras claves: Ciclofosfamida, Acrilamida, morfología espermatozoides, Análisis citogenético, Micronúcleos ratón, genotoxicidad
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Abstract
Mutagenic evaluation of the oral pills of PV-2 with in vivo assays
The oral pills PV-2 are prepared with dye of the plant Morinda royoc L., commonly named in our country like " Garañón (stud) "; what confers stimulant invigorating and stimulative effects on the libido. The development of these products has to carry out in parallel an evaluative system that allows knowing with certainty the impact that such biotechnical products have on human health, animal and the environment. It was carried out the study of the mutagenic effect in mice and rats by means of chromosomal aberration, micronucleus and sperm assays, administered by oral via, using as positive controls the acrylamide (20 mg/kg b.w), cyclophosphamide (40 mg/kg p.c) and as negative control sterile water. At the end of the treatment, the animals were sacrificed and processed to obtain the preparations according to the procedure. The results proved the non-evidence of neither clastogenic effects in mouse´s bone marrow, neither mutagenic effect on the chromosomes of rat´s bone marrow. There were not either genotoxic effects or cytotoxic ones on the spermatic cells of the mouse. These results extend the knowledge on the innocuousness of these products in their relationship to the human being.
Keywords: Cyclophosphamide, Acrylamide, sperm morphology, cytogenetic analysis, Micronucleus mouse, genotoxicity
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The oral pills PV-2 are prepared with dye of the plant Morinda royoc L., commonly named in our country like " Garañón (stud) "; what confers stimulant invigorating and stimulative effects on the libido. The development of these products has to carry out in parallel an evaluative system that allows knowing with certainty the impact that such biotechnical products have on human health, animal and the environment. It was carried out the study of the mutagenic effect in mice and rats by means of chromosomal aberration, micronucleus and sperm assays, administered by oral via, using as positive controls the acrylamide (20 mg/kg b.w), cyclophosphamide (40 mg/kg p.c) and as negative control sterile water. At the end of the treatment, the animals were sacrificed and processed to obtain the preparations according to the procedure. The results proved the non-evidence of neither clastogenic effects in mouse´s bone marrow, neither mutagenic effect on the chromosomes of rat´s bone marrow. There were not either genotoxic effects or cytotoxic ones on the spermatic cells of the mouse. These results extend the knowledge on the innocuousness of these products in their relationship to the human being.
Keywords: Cyclophosphamide, Acrylamide, sperm morphology, cytogenetic analysis, Micronucleus mouse, genotoxicity
Download PDF (258 KB)