Evaluación genotóxica del extracto oleoso de la semilla de Carapa guianensis Aublet en el ensayo de micronúcleos en ratones Balb/c.
nº39 [Octubre 12- Mayo 13] Retel16/10/2012
Daniel Francisco Arencibia Arrebola,1* Luis Alfredo Rosario Fernández,2 Livan Delgado Roche,3 Adriana Alonso Laurencio,2 Yolanda Emilia Suárez Fernández, 4 Alexis Vidal Novoa.5
1 Instituto Finlay. Habana, Cuba.
2 Instituto de Farmacia y Alimentos (IFAL-U.H). Habana, Cuba.
3 Centro de Estudios para las Investigaciones y Evaluaciones Biológicas (CIEB). Habana, Cuba.
4 Universidad Agraria de La Habana (UNAH). Mayabeque, Cuba.
5 Facultad de Biología (U.H). Habana, Cuba.
* Correspondencia a: darencibia@cecmed.sld.cu
2 Instituto de Farmacia y Alimentos (IFAL-U.H). Habana, Cuba.
3 Centro de Estudios para las Investigaciones y Evaluaciones Biológicas (CIEB). Habana, Cuba.
4 Universidad Agraria de La Habana (UNAH). Mayabeque, Cuba.
5 Facultad de Biología (U.H). Habana, Cuba.
* Correspondencia a: darencibia@cecmed.sld.cu
Resumen
El extracto oleoso de la semilla de Carapa guianensis fue evaluado recientemente manifestando grandes potencialidades como antioxidante en ensayos in vivo. El objetivo de la presente investigación fue evaluar el potencial genotóxico del extracto oleoso de la semilla de Carapa guianensis en el ensayo de micronúcleos en células de la médula ósea de ratones Balb/c. Para ello se formaron 5 grupos experimentales. Un grupo placebo (Tween 65 al 2%), tres niveles de dosis del extracto (400, 1 000 y 2 000 mg/kg), administrados por vía oral durante 14 días. Por último un grupo control positivo tratado con ciclofosfamida (CF), en dosis de 50 mg/kg por vía intraperitoneal 48 y 24 h antes de la eutanasia. Se administraron 5 animales/sexo/grupo. Después de los 14 días de administración se eutanizaron por dislocación cervical y se extrajo la médula ósea del fémur para proceder a realizar la técnica citogenética de micronúcleos. Los resultados obtenidos entre controles y tratados con el extracto no difirieron para los dos sexos en las variables EP/EN como índice de citotoxicidad y el % de EP con micronúcleos como índice de daño genotóxico. Sin embargo si difirieron controles y tratados contra el grupo tratado con CF, validando nuestros resultados. Se concluye que el extracto oleoso de la semilla de la Carapa guianensis no posee potencialidades genotóxicas en la formación de micronúcleos en células de la médula ósea de ratones Balb/c de ambos sexos.
Palabras claves: Extracto oleoso, Carapa guianensis, micronúcleos, ratones Balb/c.
Descargar Archivo PDF (192 KB)
Palabras claves: Extracto oleoso, Carapa guianensis, micronúcleos, ratones Balb/c.
Descargar Archivo PDF (192 KB)
Abstract
Genotoxic assessment of the Carapa guianensis Aublet seed oleaginous extract in Balb/c mice micronucleus assay.
The seed oil extract of Carapa guianensis has various biomedical applications. Recently was evaluated expressing great potential as in vivo antioxidant assays. The aimed of this study was to evaluate the genotoxic potential of the seed oil Carapa guianensis extract in the micronucleus assay of bone marrow cells in Balb/c mice. We were formed 5 experimental groups. A placebo group (Tween 65, 2%), three dose levels of the extract (400, 1 000 and 2 000 mg/kg) administered orally for 14 days. Finally a positive control group treated with cyclophosphamide (CF), at a dose of 50 mg/kg intraperitoneally 48 and 24 h before euthanasia. Were administered 5 animals/sex/group. After 14 days of dosing the animals were euthanized by cervical dislocation and the bone marrow of femur was extracted to proceed with the micronucleus cytogenetic technique. The results between controls and treated with the extract did not differ for the two sexes in the cytotoxicity index (PE/NE) and the PE percent with micronucleus as genotoxicity index. However the controls and treated with the extract were differed from the group treated with CF, validating our results. We conclude that the oleaginous extract of the Carapa guianensis seeds has not genotoxic potential in the formation of micronucleus in Balb/c mice bone marrow of both sexes.
Key words: Oleaginous extract, Carapa guianensis, micronucleus, Balb/c mice.
Download PDF (192 KB)
The seed oil extract of Carapa guianensis has various biomedical applications. Recently was evaluated expressing great potential as in vivo antioxidant assays. The aimed of this study was to evaluate the genotoxic potential of the seed oil Carapa guianensis extract in the micronucleus assay of bone marrow cells in Balb/c mice. We were formed 5 experimental groups. A placebo group (Tween 65, 2%), three dose levels of the extract (400, 1 000 and 2 000 mg/kg) administered orally for 14 days. Finally a positive control group treated with cyclophosphamide (CF), at a dose of 50 mg/kg intraperitoneally 48 and 24 h before euthanasia. Were administered 5 animals/sex/group. After 14 days of dosing the animals were euthanized by cervical dislocation and the bone marrow of femur was extracted to proceed with the micronucleus cytogenetic technique. The results between controls and treated with the extract did not differ for the two sexes in the cytotoxicity index (PE/NE) and the PE percent with micronucleus as genotoxicity index. However the controls and treated with the extract were differed from the group treated with CF, validating our results. We conclude that the oleaginous extract of the Carapa guianensis seeds has not genotoxic potential in the formation of micronucleus in Balb/c mice bone marrow of both sexes.
Key words: Oleaginous extract, Carapa guianensis, micronucleus, Balb/c mice.
Download PDF (192 KB)