Efectos tóxicos de la sacarosa e ion amonio, en larvas de rana Osteopilus septentrionalis (Anura: Ranidae)
n°12 [mar-may 2007] RetelTrujillo Hernández. J, Carballo Hondal. O, Castañeda León. J.M, Álvarez Pérez. E, Reyes González A. Centro Nacional de Toxicología
Resumen
Palabras claves: sacarosa, ion amonio, larvas de rana,
toxicidad, Osteopilus septentrionalis
Abstract
Toxic Effects of Saccharosa and Ammonium Ion in Osteopilus
septentrionalis larvae.
The culture media used in the production of bioproducts for
agricultural purposes are rich in carbon hydrates. There are
some organisms unable to assimilate these carbon hydrates.
Formulations containing ammonium ions are potentially toxic.
The objectives of this work are to record sings of toxicity
or mortality in larvae of frogs (Osteospilus septentrionalis),
macroscopic and histological damages in organs and tissues
of larvae exposed to the culture medium and to ammonium sulphate.
A static 96 hours study was carried out with healthy larvae,
between the 25 – 26 stagets according to Gosner classification.
The concentrations evaluated were: 0.1, 1.0, 10.0 and 100.0
mg/l for both culture medium and Ammonium Sulphate. Concentrations
were calculated according to their proportion in the final
formulation. Only under the 100.0 mg/l concentration, the
100 % of larvae died after 96 hours. Both groups showed signs
of toxicity, evidenced by injury in organs and tissues (liver,
vesicle, pancreas and intestines). An excessive ingestion
of carbon hydrates (saccharosa) caused cell degeneration in
liver and pancreas cells, this finding coincided with the
reports of other authors. It is concluded that the excessive
ingestion of saccharosa and ammonium caused death and damages
to the frog larvae Osteospilus septentrionalis kept under
pond conditions.
Keywords: saccharosa, ammonium ions, larvae or frogs,
toxicity, Osteopilus septentrionalis