Efectos de la Ciclofosfamida en la morfología del espermatozoide de ratón
nº34 [noviembre 10 - febrero 11] Retel28/12/2010
Ailemys Curbelo Valiente, Yesenia Rivero Salgado, Niurka Somoza Pérez, María Elena Arteaga Pérez, Nereida Mantilla Gattorno.
Centro Nacional para la Producción de Animales de Laboratorio, CENPALAB. Finca Tirabeque, Carretera Cacahual Km 21/2, Bejucal, La Habana Cuba. Correo: ailemys@cenpalab.inf.cu, ailemyscurbelo@yahoo.es
Resumen
La Ciclofosfamida (Cf) es un agente alquilante comúnmente utilizada como fármaco anti-cáncer e inmunosupresor. Es usado en el tratamiento de leucemia crónica y aguda, mielomas múltiples, linfomas, artritis reumáticas, trasplante de médula ósea, entre otras enfermedades. Se utiliza como control positivo en diferentes ensayos de mutagénesis. En este estudio fueron usadas dos dosis (50 y 100 mg/Kg) de Cf con el objetivo de determinar su capacidad de inducir anormalidades en el espermatozoide del ratón NMRI. El Ensayo de Anormalidades de la Cabeza del Espermatozoide, descrito por Wyrobek y Bruce en 1975 propone un sistema para detectar daño citogenético en células germinales. Permite cuantificar poblaciones de espermatozoides anormales y constituye una prueba in vivo simple y rápida. La frecuencia de cabezas anormales analizadas (cabezas amorfas, banana y sin gancho) aumentó significativamente en el tratamiento con 100 mg/Kg de Cf, estos resultados evidencian que dicha sustancia provoca daño genotóxico en células germinales de ratón.
Palabras claves: Ciclofosfamida, morfología espermatozoides, ratón, genotoxicidad
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Palabras claves: Ciclofosfamida, morfología espermatozoides, ratón, genotoxicidad
Descargar Archivo PDF (148 KB)
Abstract
Effects of a ciclophosphamide on mouse sperm morphology.
Cyclophosphamide (CP) as an alkylating agent is the most commonly used anticancer and immunosuppressant drug. It is used for the treatment of chronic and acute leukemia, multiple myeloma, lymphomas, rheumatic arthritis, bone marrow transplantation, among other diseases. It is used as positive control in different mutagenesis tests. In this study, they were used two doses (50 y 100 mg/Kg) of CP, with the objective to determinate the induced-abnormality capacity in the NMRI mouse sperm. The Morphology of the Head of the Sperm Cell Test, described by Wyrobek and Bruce in 1975, proposed a system to detect cytogenetic damage in germ cells. It permits to quantify abnormal sperm populations and constitutes a single and rapid in vivo test. The frequency of abnormal heads (amorphous head, banana-shape and no hook) increased significantly in the 100 mg/Kg CP, this results evidence that this substance induces genotoxic damage in mouse germ cells.
Keywords: Cyclophosphamide, sperm morphology, mouse, genotoxicity
Download PDF (148 KB)
Cyclophosphamide (CP) as an alkylating agent is the most commonly used anticancer and immunosuppressant drug. It is used for the treatment of chronic and acute leukemia, multiple myeloma, lymphomas, rheumatic arthritis, bone marrow transplantation, among other diseases. It is used as positive control in different mutagenesis tests. In this study, they were used two doses (50 y 100 mg/Kg) of CP, with the objective to determinate the induced-abnormality capacity in the NMRI mouse sperm. The Morphology of the Head of the Sperm Cell Test, described by Wyrobek and Bruce in 1975, proposed a system to detect cytogenetic damage in germ cells. It permits to quantify abnormal sperm populations and constitutes a single and rapid in vivo test. The frequency of abnormal heads (amorphous head, banana-shape and no hook) increased significantly in the 100 mg/Kg CP, this results evidence that this substance induces genotoxic damage in mouse germ cells.
Keywords: Cyclophosphamide, sperm morphology, mouse, genotoxicity
Download PDF (148 KB)